RNA extraction and leptin receptor mRNA detection in bull ejaculated spermatozoa

Detection of leptin and leptin receptor mRNA expression in the bovine t

Leptin receptor mRNA expression in Taleshi ram gonads

Leptin is secreted mainly by fat, which is involved in energy metabolism and reproduction. Leptin and itsreceptor (Ob-R) have been detected in human spermatozoa and testis, thus it can be concluded leptin involves in male physiology. The goal of this study was determination of the presence of leptin receptor mRNA in the ram testis, epididymis and spermatozoa by reverse transcription polymerase ...

leptin receptor mrna expression in taleshi ram gonads

leptin is secreted mainly by fat, which is involved in energy metabolism and reproduction. leptin and itsreceptor (ob-r) have been detected in human spermatozoa and testis, thus it can be concluded leptin involves in male physiology. the goal of this study was determination of the presence of leptin receptor mrna in the ram testis, epididymis and spermatozoa by reverse transcription polymerase ...

The effect of ouabain on membrane potential and flagellar wave in ejaculated bull spermatozoa.

Ejaculated bull spermatozoa were exposed to various concentrations of ouabain to ascertain the effect on membrane potential, intracellular concentrations of sodium and potassium and motility. Membrane potenital, measured by electrophysiological methods, decreased. Intracellular potassium decreased and intracellular sodium increased. Progressive motility decreased. In addition, the motility chan...

Effects of carnitine on oxygen uptake and utilization of (U-14C)palmitate by ejaculated bull spermatozoa.

Fatty acid oxidation by washed intact ejaculated bull spermatozoa was depressed by carnitine concentrations as low as 5 mM, whereas oxygen uptake was only depressed by concentrations of 20 mM or above. Incorporation of (U-14C)-palmitate into 1,2-diglycerides was stimulated, and there was some stimulation of incorporation into phospholipids.

Purification and characterization of two plasma membrane domains from ejaculated bull spermatozoa

Plasma membranes were detached from ejaculated bull spermatozoa by a brief sonication in a moderately hypotonic medium, and the released plasma membranes were partially purified by differential centrifugation. The resulting fraction was enriched 8- and 15-fold in alkaline phosphatase and 5' nucleotidase activities, respectively, compared with the starting sonicated spermatozoa. This total plasm...